The popular Peterborough club on Broadway has been open for sit-down bookings since Prime Minister Boris Johnson announced that pubs could open again on July 4.
However, following the latest government guidelines, as the venue opens at 5pm, they have announced they will be closing again.
They wrote on their social media: "Due to the recent government announcement we have taken the decision to close our doors.
"We would like to thank our customers for supporting us through this difficult time and look forward to welcoming you back soon."
The club has continued to get bookings since they reopened in July, and after successfully welcoming people back into Embassy, they also reopened Flares, both for table bookings only, with the dance floor closed.
At the time, they reassured customers that they had put their best efforts in to make it as Covid-19 secure as possible. Social distancing measures, cleaning rules and an order and pay app were introduced.
New rules were announced on Tuesday, September 22, by Prime Minister Boris Johnson in response to coronavirus numbers increasing.
From today, September 24, pubs, bars and restaurants will be table-service only and hospitality, leisure and entertainment venues will be subject to a 10pm closing time. Takeaways will also close from 10pm to 5am, although they will be allowed to deliver.
Mr Johnson said: "While the vast majority have complied with the rules, there have been too many breaches – too many opportunities for our invisible enemy to slip through undetected.
"The virus has stated to spread again in an exponential way. Infections are up, hospital admissions are climbing."
"If we follow these simple rules together, we will get through this winter together," he said. "There are unquestionably difficult months to come.
"And the fight against Covid-19 is by no means over. I have no doubt, however, that there are great days ahead."
Mr Johnson warned that these rules could be in place for six months, depending on the progress of vaccines, treatments and tests.